The Czech Republic demanded on Wednesday that Russia should allow its expelled diplomats to return to Moscow, threatening that otherwise more Russian diplomats would be asked to leave Prague.

The Czech request could further escalate a diplomatic conflict with Russia over the alleged involvement of Russian spies in a massive ammunition depot explosion.

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Car of Russia's ambassador to Prague, Aleksandr Zmeyevsky is parked in front of the Foreign Ministry in Prague, Czech Republic, Wednesday, April 21, 2021. The ambassador was summoned by newly appointed Foreign Minister Kulhanek to be handed the Czech protest against the Russian expulsion move, which the Czechs consider disproportionate, saying it has paralyzed the Czech Embassy in Moscow following the fierce diplomatic crisis over allegations that Russian agents were involved in a massive Czech ammunition depot explosion. (AP PhotoPetr David Josek)

The Czech Republic demanded on Wednesday that Russia should allow its expelled diplomats to return to Moscow, threatening that otherwise more Russian diplomats would be asked to leave Prague.

Czech Republic's Prime Minister Andrej Babis, right, and newly appointed Foreign Minister Jakub Kulhanek address media at the Cernin's Palace in Prague, Czech Republic, Wednesday, April 21, 2021. Kulhanek was appointed during a Czech Russia diplomatic crisis over the alleged involvement in a fatal ammunition depot explosion in 2014. (AP PhotoPetr David Josek)

The new Czech Foreign Minister, Jakub Kulhanek, summoned Russian ambassador to Prague Aleksandr Zmeyevsky to protest what he called Russia's “inappropriate reaction.”

Czech diplomats and their families arrive at the Vaclav Havel airport in Prague, Czech Republic, Monday, April 19, 2021. Russia has ordered 20 Czech diplomats to leave the country within a day in response to Prague's expulsion of 18 Russian diplomats. The Czech government has alleged the Russian Embassy staffers were spies for a military intelligence agency that was involved in a fatal ammunition depot explosion in 2014. (AP PhotoPetr David Josek)

Kulhanek gave the Russians a Thursday noon deadline “to enable the return of all (Czech) expelled diplomats to the Czech embassy in Moscow.”

Czech diplomats and their families arrive at the Vaclav Havel airport in Prague, Czech Republic, Monday, April 19, 2021. Russia has ordered 20 Czech diplomats to leave the country within a day in response to Prague's expulsion of 18 Russian diplomats. The Czech government has alleged the Russian Embassy staffers were spies for a military intelligence agency that was involved in a fatal ammunition depot explosion in 2014. (AP PhotoPetr David Josek)

“Czech-Russian relations have entered an extraordinary difficult phase and I, as a foreign minister, am not happy about it,” he said.

Czech diplomats and their families arrive at the Vaclav Havel airport in Prague, Czech Republic, Monday, April 19, 2021. Russia has ordered 20 Czech diplomats to leave the country within a day in response to Prague's expulsion of 18 Russian diplomats. The Czech government has alleged the Russian Embassy staffers were spies for a military intelligence agency that was involved in a fatal ammunition depot explosion in 2014. (AP PhotoPetr David Josek)

The same two Russians were charged by British authorities in absentia in 2018 with trying to kill former Russian spy Sergei Skripal and his daughter with the Soviet-era nerve agent Novichok in the English city of Salisbury.

It came after Russia ordered 20 Czech diplomats to leave the country on Sunday, in retaliation for the Czech government’s expulsion of 18 Russian diplomats it identified as spies from the GRU and the SVR, Russia’s military and foreign intelligence services.

Car of Russia's ambassador to Prague, Aleksandr Zmeyevsky is parked in front of the Foreign Ministry in Prague, Czech Republic, Wednesday, April 21, 2021. The ambassador was summoned by newly appointed Foreign Minister Kulhanek to be handed the Czech protest against the Russian expulsion move, which the Czechs consider disproportionate, saying it has paralyzed the Czech Embassy in Moscow following the fierce diplomatic crisis over allegations that Russian agents were involved in a massive Czech ammunition depot explosion. (AP PhotoPetr David Josek)

Car of Russia's ambassador to Prague, Aleksandr Zmeyevsky is parked in front of the Foreign Ministry in Prague, Czech Republic, Wednesday, April 21, 2021. The ambassador was summoned by newly appointed Foreign Minister Kulhanek to be handed the Czech protest against the Russian expulsion move, which the Czechs consider disproportionate, saying it has paralyzed the Czech Embassy in Moscow following the fierce diplomatic crisis over allegations that Russian agents were involved in a massive Czech ammunition depot explosion. (AP PhotoPetr David Josek)

The new Czech Foreign Minister, Jakub Kulhanek, summoned Russian ambassador to Prague Aleksandr Zmeyevsky to protest what he called Russia's “inappropriate reaction.”

Kulhanek said that because of the high number of staffers at the Russian embassy in Prague, the Czech action “by no means” threatens its normal functioning.

But for the Czechs, the Russian move “paralyzed" their Moscow embassy.

Czech Republic's Prime Minister Andrej Babis, right, and newly appointed Foreign Minister Jakub Kulhanek address media at the Cernin's Palace in Prague, Czech Republic, Wednesday, April 21, 2021. Kulhanek was appointed during a Czech Russia diplomatic crisis over the alleged involvement in a fatal ammunition depot explosion in 2014. (AP PhotoPetr David Josek)

Czech Republic's Prime Minister Andrej Babis, right, and newly appointed Foreign Minister Jakub Kulhanek address media at the Cernin's Palace in Prague, Czech Republic, Wednesday, April 21, 2021. Kulhanek was appointed during a Czech Russia diplomatic crisis over the alleged involvement in a fatal ammunition depot explosion in 2014. (AP PhotoPetr David Josek)

Kulhanek gave the Russians a Thursday noon deadline “to enable the return of all (Czech) expelled diplomats to the Czech embassy in Moscow.”

If not, he warned, “I will decide tomorrow afternoon to reduce the staff of the Russian embassy in Prague so their number equals the current number of staff at the Czech embassy in Moscow.”

Kulhanek said he agreed the move with Prime Minister Andrej Babis and President Milos Zeman, who is known for his pro-Russian views.

Czech diplomats and their families arrive at the Vaclav Havel airport in Prague, Czech Republic, Monday, April 19, 2021. Russia has ordered 20 Czech diplomats to leave the country within a day in response to Prague's expulsion of 18 Russian diplomats. The Czech government has alleged the Russian Embassy staffers were spies for a military intelligence agency that was involved in a fatal ammunition depot explosion in 2014. (AP PhotoPetr David Josek)

Czech diplomats and their families arrive at the Vaclav Havel airport in Prague, Czech Republic, Monday, April 19, 2021. Russia has ordered 20 Czech diplomats to leave the country within a day in response to Prague's expulsion of 18 Russian diplomats. The Czech government has alleged the Russian Embassy staffers were spies for a military intelligence agency that was involved in a fatal ammunition depot explosion in 2014. (AP PhotoPetr David Josek)

“Czech-Russian relations have entered an extraordinary difficult phase and I, as a foreign minister, am not happy about it,” he said.

Czech leaders said on Saturday they have evidence provided by the intelligence and security services that points to the participation of two agents of GRU’s elite Unit 29155 in the 2014 blast that killed two.

Russia denied that.

Czech diplomats and their families arrive at the Vaclav Havel airport in Prague, Czech Republic, Monday, April 19, 2021. Russia has ordered 20 Czech diplomats to leave the country within a day in response to Prague's expulsion of 18 Russian diplomats. The Czech government has alleged the Russian Embassy staffers were spies for a military intelligence agency that was involved in a fatal ammunition depot explosion in 2014. (AP PhotoPetr David Josek)

Czech diplomats and their families arrive at the Vaclav Havel airport in Prague, Czech Republic, Monday, April 19, 2021. Russia has ordered 20 Czech diplomats to leave the country within a day in response to Prague's expulsion of 18 Russian diplomats. The Czech government has alleged the Russian Embassy staffers were spies for a military intelligence agency that was involved in a fatal ammunition depot explosion in 2014. (AP PhotoPetr David Josek)

The same two Russians were charged by British authorities in absentia in 2018 with trying to kill former Russian spy Sergei Skripal and his daughter with the Soviet-era nerve agent Novichok in the English city of Salisbury.

Kulhanek made the statement just hours after he was sworn in Wednesday.

Before his meeting with the Russian ambassador, he consulted NATO Secretary-General Jens Stoltenberg and thanked him for his support “in these uneasy times.” The Czech Republic — once part of Soviet Russian-controlled communist eastern Europe — is now a NATO member.

Czech diplomats and their families arrive at the Vaclav Havel airport in Prague, Czech Republic, Monday, April 19, 2021. Russia has ordered 20 Czech diplomats to leave the country within a day in response to Prague's expulsion of 18 Russian diplomats. The Czech government has alleged the Russian Embassy staffers were spies for a military intelligence agency that was involved in a fatal ammunition depot explosion in 2014. (AP PhotoPetr David Josek)

Czech diplomats and their families arrive at the Vaclav Havel airport in Prague, Czech Republic, Monday, April 19, 2021. Russia has ordered 20 Czech diplomats to leave the country within a day in response to Prague's expulsion of 18 Russian diplomats. The Czech government has alleged the Russian Embassy staffers were spies for a military intelligence agency that was involved in a fatal ammunition depot explosion in 2014. (AP PhotoPetr David Josek)